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Which of the following is not a condition for PCR?

Filza

16 Jun, 2022

A. Initial melting carried out for 5 minutes at 94 degrees

B. Initial melting followed by 30 cycles each consisting of melting for 1 minute at 94 degrees

C. Renaturation for 5 minutes at 60 degrees

D. DNA synthesis at 72 degrees for 1.5 minutes

Explanation: The melting temperature is 94 degrees and the initial melting is carried for 5 minutes at this temperature. It is followed by 30 cycles each consisting of melting for 1 minute at 94 degrees. The renaturation is carried for 1 minute at 60 degrees. The DNA synthesis is carried out at 72 degrees for 1.5 minutes. After the 30 cycles, a final round of extension is carried out for 10 minutes.

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