These are steps taken in carrying out the PCR reaction: i) Attachment of primers by cooling ii) Denaturation of strands iii) DNA synthesis iv) Heating Which is the correct order?

A. i)-ii)-iii)-iv)

B. ii)-i)-iii)-iv)

C. iv)-iii)-ii)-i)

D. iv)-ii)-i)-iii)

Explanation: PCR consists of a series of steps. Firstly, the reaction mixture is heated so that the strands are separated i.e. their denaturation takes place. Then it is again cooled so that the primers are able to attach. Once the primers are attached, the synthesis of DNA is allowed. This whole process is repeated.