Which of the following orientation is not propagated to later stages?

A. Circular plasmids having genomic DNA

B. Intramolecular ligation of plasmids

C. Linear molecules

D. Head-to-head ligation of two DNA plasmids

Explanation: When a cloning experiment is carried out, there are various orientations that are possible. The orientation which is not propagated to the later stages is a linear molecule. Other possible orientations are relegation of plasmid and intramolecular ligation of genomic DNA. Intermolecular ligation of two DNA plasmids and genomic plasmids is also possible.