Which of the following activity is not present in Taq polymerase?

A. 5’-3’ polymerase

B. 5’-3’ exonuclease

C. 3’-5’ exonuclease

D. Both 5’-3’ polymerase and 5’-3’ exonuclease

Explanation: Taq polymerase is not having 3’-5’ exonuclease activity. It is a proof reading activity and it is very important to have a check on the mutations if they are encountered in the PCR products.

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