PCR products can be analysed in many ways. Which of the following is not possible?

A. Use of restriction enzymes

B. Determining whether a particular oligonucleotide probe hybridizes to a PCR product

C. Electrophoresis

D. Direct sequencing can’t be carried out

Explanation: Restriction enzymes can be used and analyses can be done via destroying the restriction site. Checking for the hybridization of oligonucleotide probe with a PCR product. Electrophoresis can be used to check the mobility and compare it with a wild type molecule. Direct sequencing can also be carried out in order to analyse the PCR product.