Often PCR can be performed in order to confirm whether an insert is present in the plasmid. Cells are taken directly and PCR is performed, this type of PCR is known as __________

A. direct PCR

B. colony PCR

C. quantitative PCR

D. in-situ PCR

Explanation: When cells from colonies are taken and direct PCR is performed, it is called as colony PCR. Primers anneal to each side of the cloning site and there is no need for purification in such cases.