If a plasmid is having two antibiotic resistant genes, say ampicillin resistant and chloramphenicol resistant. If the plasmid grows in ampicillin containing medium but not in chloramphenicol, what can be concluded?

A. The insert is not present in any of the genes

B. The insert is present in the ampicillin gene but not in the chloramphenicol gene

C. The insert is present in the chloramphenicol gene but not in the ampicillin gene

D. The insert is present between both of the genes

Explanation: The insert is present in the chloramphenicol gene and not in the ampicillin gene. As the insert is present in it, it disrupts its function and hence they are not able to grow in chloramphenicol containing a medium.