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A reaction mixture for PCR consists of ____________.

Filza

16 Jun, 2022

A. heat unstable polymerase

B. primers in a limited amount

C. deoxynucleoside triphosphate (dNTPs)

D. a region complementary to the sequence to be amplified

Explanation: A reaction mixture for PCR consists of the region of the DNA sequence to be amplified, primers in large molar excess, heat stable polymerase and dNTPs. Heat stable polymerase which is used commonly is Taq polymerase.

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